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KMID : 1123920060200051321
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Korean Journal of Oriental Physiology and Pathology
2006 Volume.20 No. 5 p.1321 ~ p.1326
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Purification of Total Ginsesides with Macroporous Resins and Their Biological Activities
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Li Huayue
Jin Haizhu
Lee Dong-Geun
Lee Jae-Hwa
Lee Sang-Hyeon
Ha Bae-Jin
Ha Jong-Myung
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Abstract
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Total ginsenosides were purified and their antioxidant, antibacterial and anticancer activities were measured. The crude extracts of ginseng, which were extracted with 75% ethanol by ultrasonification method, were firstly purified on AB-8 macroporous adsorption column to remove water soluble impurities, and decolored on Amberlite IRA 900 Cl anion-exchange column. Then, they were purified on Amberlite XAD16 adsorption column to delete the non-polar impurities. Total ginsenosides contents of the purified extracts were 79.4%, 71.7% and 72.5% in cultured wild ginseng, red ginseng and white ginseng, which were significantly increased than those of crude extracts. All of the three extracts showed concentration-dependant scavenging activities against DPPH radicals, among which white ginseng showed the most powerful activity. Cultured wild ginseng roots showed strongest effect against both B. subtilis PM 125(Gram-positive) and E. coli D31 (Gram-negative) bacteria, while red ginseng and white ginseng only showed the activity against B. subtilis. According to the result of the MTT assay, ail of the three extracts inhibited the growth of U-937 human hohistiocytic lympma cell, which were significantly different (p < 0.05) when compared to the control.
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KEYWORD
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ginsenoside, macroporous resin, purification, antioxidant, antibacterial, anticancer
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